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The aim of the course is to introduce students to the discipline of genotoxicology. There are several physical, biological and mostly chemical risk factors in our environment that can harm health by damaging the DNA. For the comprehension of this problem it is important to know the main types and causal factors of DNA damage the characterization of which can be done by genotoxicological tests. The theoretical background and usability of these test will be explained with detailed discussion of the bacterial Salmonella typhimurium reverse mutation test (Ames test), assessment of chromosomal aberrations, sister chromatid exchange, micronucleus test and the single-cell gel electrophoresis assay (comet assay) that is capable of detecting single- and double-strand breaks and alkali labile sites. The students can practice the assessment of chromosomal aberrations under microscope and the process of comet assay from the preparation of cells to the computer-based analysis of results.
The lectures will be available from the starting date (23 March 2020) and are to be processed individually. The laboratory practical will be held at a pre-specified time when student entry restrictions will be over. The evaluation of the course will be based on the completion of the laboratory exercise.